Genome Editing and Cardiac Regeneration

Rayhaan Bassawon; Kashif Khan; Ateeque Siddique; Dominique Shum-Tim

doi:10.1007/978-981-19-5642-3_3

Genome Editing and Cardiac Regeneration

Adv Exp Med Biol. 2023:1396:37-52. doi: 10.1007/978-981-19-5642-3_3.

Authors

Rayhaan Bassawon¹, Kashif Khan², Ateeque Siddique^{2

3}, Dominique Shum-Tim^{4

5

6}

Affiliations

¹ Division of Cardiac Surgery, Royal Victoria Hospital, McGill University Health Centre, Montreal, QC, Canada.
² Division of Cardiology and Cardiac Surgery, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada.
³ Department of Experimental Surgery, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada.
⁴ Division of Cardiac Surgery, Royal Victoria Hospital, McGill University Health Centre, Montreal, QC, Canada. [email protected].
⁵ Division of Cardiology and Cardiac Surgery, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada. [email protected].
⁶ Department of Experimental Surgery, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada. [email protected].

PMID: 36454458
DOI: 10.1007/978-981-19-5642-3_3

Abstract

Although the field of cardiac regeneration is relatively young, it is progressing rapidly with technological advancements. Genome editing tools are allowing researchers to creatively influence signaling pathways to be able to shed light on them and are important for addressing certain issues and limitations associated with in vitro and in vivo aspects of cardiac regeneration, such as imaging and immune rejection. In this chapter, the pathways involved in cardiac regeneration will be highlighted, and the role of gene-editing tools in endogenous and exogenous approaches to regenerate injured myocardium is discussed.

Keywords: Adeno-associated virus; CRISPR/Cas9; Cardiomyocytes; Ischemic heart disease; iPSCs.

MeSH terms

Gene Editing*
Heart*
Humans
Myocardium
Research Personnel