Serum anti-GM2 and anti-GalNAc-GD1a ganglioside IgG antibodies are biomarkers for immune-mediated polyneuropathies in cats

J Peripher Nerv Syst. 2023 Mar;28(1):32-40. doi: 10.1111/jns.12529. Epub 2023 Jan 16.

Abstract

Recent work identified anti-GM2 and anti-GalNAc-GD1a IgG ganglioside antibodies as biomarkers in dogs clinically diagnosed with acute canine polyradiculoneuritis, in turn considered a canine equivalent of Guillain-Barré syndrome. This study aims to investigate the serum prevalence of similar antibodies in cats clinically diagnosed with immune-mediated polyneuropathies. The sera from 41 cats clinically diagnosed with immune-mediated polyneuropathies (IPN), 9 cats with other neurological or neuromuscular disorders (ONM) and 46 neurologically normal cats (CTRL) were examined for the presence of IgG antibodies against glycolipids GM1, GM2, GD1a, GD1b, GalNAc-GD1a, GA1, SGPG, LM1, galactocerebroside and sulphatide. A total of 29/41 IPN-cats had either anti-GM2 or anti-GalNAc-GD1a IgG antibodies, with 24/29 cats having both. Direct comparison of anti-GM2 (sensitivity: 70.7%; specificity: 78.2%) and anti-GalNAc-GD1a (sensitivity: 70.7%; specificity: 70.9%) antibodies narrowly showed anti-GM2 IgG antibodies to be the better marker for identifying IPN-cats when compared to the combined ONM and CTRL groups (P = .049). Anti-GA1 and/or anti-sulphatide IgG antibodies were ubiquitously present across all sample groups, whereas antibodies against GM1, GD1a, GD1b, SGPG, LM1 and galactocerebroside were overall only rarely observed. Anti-GM2 and anti-GalNAc-GD1a IgG antibodies may serve as serum biomarkers for immune-mediated polyneuropathies in cats, as previously observed in dogs and humans.

Keywords: (max 5): feline; GM2; auto-antibody; glycolipid; poly(radiculo)neuropathy.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoantibodies
  • Biomarkers
  • Cats
  • Dogs
  • G(M1) Ganglioside
  • G(M2) Ganglioside
  • Galactosylceramides
  • Gangliosides
  • Guillain-Barre Syndrome*
  • Humans
  • Immunoglobulin G
  • Polyneuropathies* / diagnosis
  • Polyneuropathies* / veterinary

Substances

  • ganglioside, GD1a
  • galactocerebroside
  • Galactosylceramides
  • G(M1) Ganglioside
  • sulfate-3-glucuronyl paragloboside
  • Gangliosides
  • Immunoglobulin G
  • Biomarkers
  • Autoantibodies
  • G(M2) Ganglioside