Single-cell biological network inference using a heterogeneous graph transformer

Nat Commun. 2023 Feb 21;14(1):964. doi: 10.1038/s41467-023-36559-0.

Abstract

Single-cell multi-omics (scMulti-omics) allows the quantification of multiple modalities simultaneously to capture the intricacy of complex molecular mechanisms and cellular heterogeneity. Existing tools cannot effectively infer the active biological networks in diverse cell types and the response of these networks to external stimuli. Here we present DeepMAPS for biological network inference from scMulti-omics. It models scMulti-omics in a heterogeneous graph and learns relations among cells and genes within both local and global contexts in a robust manner using a multi-head graph transformer. Benchmarking results indicate DeepMAPS performs better than existing tools in cell clustering and biological network construction. It also showcases competitive capability in deriving cell-type-specific biological networks in lung tumor leukocyte CITE-seq data and matched diffuse small lymphocytic lymphoma scRNA-seq and scATAC-seq data. In addition, we deploy a DeepMAPS webserver equipped with multiple functionalities and visualizations to improve the usability and reproducibility of scMulti-omics data analysis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Benchmarking*
  • Cluster Analysis
  • Data Analysis*
  • Electric Power Supplies
  • Reproducibility of Results
  • Single-Cell Analysis

Associated data

  • figshare/10.6084/m9.figshare.c.5018987.v1
  • figshare/10.6084/m9.figshare.5968960.v1