Objective: To investigate the association between the expression of long non-coding RNA genes and the HULC rs7763881 polymorphism, recurrence, and metastasis after radical resection in patients with hepatocellular carcinoma (HCC). Methods: Paraffin tissue samples were selected from 426 cases diagnosed with HCC between January 2004 to January 2012. The expression of different genotypes of HULC gene locus rs7763881 in paraffin tissues was detected by PCR, and the association between different genotype expressions and clinical case characteristics of HCC [gender, age, TNM stage, alpha-fetoprotein, tumor maximum diameter (cm), vascular invasion, tumor capsule, tumor grade] was analyzed. Cox proportional risk regression model was used to analyze the correlation between different genotypes and clinicopathological features, prognosis, and recurrence. Survival analysis between different genotypes was performed using the Kaplan-Meier method for a parallel log-rank test. Results: There were 27 (6.3%) cases in the whole group who lost to follow-up. A total of 399 (93.7%) specimens were included in the study, and 105 (26.3%), 211 (52.9%) and 83 (20.8%) were included in the rs77638881 AA, AC, and CC genotypes, respectively. Kaplan-Meier curve showed that the postoperative overall survival and recurrence-free survival rate were significantly higher in patients with the AA than AC/CC genotype (P < 0.05). Univariate analysis showed that the AC/CC genotype was closely related to tumor vascular invasion and recurrence or metastasis of HCC (P < 0.05). Cox multivariate analysis results showed that patients with the AA genotype were taken as references, and the results showed that the risk of recurrence and metastasis in patients with the CA/CC genotype increased to varying degrees, with statistical significance (P < 0.05). Conclusion: The rs7763881 polymorphic loci located on the HULC gene are closely related to HCC recurrence and metastasis after radical resection. Thus, it may be an indicator for evaluating HCC recurrence and metastasis.
目的： 探讨肝细胞癌（HCC）患者中肝癌高表达长链非编码RNA（HULC）基因位点rs7763881多态性的表达及其与根治术后复发转移的关系。 方法： 选取2004年1月到2012年1月426例确诊为HCC的石蜡组织样本，采用PCR方法检测肝组织中HULC基因位点rs7763881的不同基因型表达，分析不同的基因型表达与肝癌临床病例特征[性别、年龄、TNM分期、甲胎蛋白、肿瘤最大直径（cm）、血管侵犯、肿瘤包膜、肿瘤分级]的关系；采用Cox比例风险回归模型多因素分析不同基因分型、不同临床病理特征的基因分型与预后及复发的相关性；不同基因分型之间的生存分析采用Kaplan Meier法并行log-rank检验。 结果： 全组病例有27例（6.3%）失访，共399例（93.7%）标本纳入研究，rs7763881位点AA、AC和CC基因型分别有105例（26.3%）、211例（52.9%）和83例（20.8%）。Kaplan–Meier曲线显示携带AA基因型的患者术后总生存率和无复发生存率明显高于携带AC/CC基因型的患者（P<0.05）。单因素分析显示AC/CC基因型与HCC的肿瘤血管侵犯以及复发或转移密切相关（P<0.05）；Cox多因素分析结果显示以AA基因型患者为参考，结果显示携带CA/CC基因型的患者复发转移风险均有不同程度增加，差异具有统计学意义（P<0.05）。 结论： HULC基因位点rs7763881多态性与HCC根治术后复发转移密切相关，可能为评估HCC复发、转移的指标。.
Keywords: Gene, HULC; Hepatocellular carcinoma; Prognosis; Single nucleotide polymorphism; rs7763881.