Implementation of circulating tumour DNA multi-target mutation testing in plasma: a perspective from an external quality assessment providers' survey

Virchows Arch. 2024 Oct;485(4):717-722. doi: 10.1007/s00428-023-03558-x. Epub 2023 May 19.

Abstract

Demand for large-scale tumour profiling across cancer types has increased in recent years, driven by the emergence of targeted drug therapies. Analysing alternations in plasma circulating tumour DNA (ctDNA) for cancer detection can improve survival; ctDNA testing is recommended when tumour tissue is unavailable. An online survey of molecular pathology testing was circulated by six external quality assessment members of IQN Path to registered laboratories and all IQN Path collaborative corporate members. Data from 275 laboratories across 45 countries were collected; 245 (89%) perform molecular pathology testing, including 177 (64%) which perform plasma ctDNA diagnostic service testing. The most common tests were next-generation sequencing-based (n = 113). Genes with known stratified treatment options, including KRAS (n = 97), NRAS (n = 84), and EGFR (n = 130), were common targets. The uptake of ctDNA plasma testing and plans to implement further testing demonstrates the importance of support from a well-designed EQA scheme.

Keywords: Circulating tumour DNA; EQA scheme; Large-scale tumour profiling.

MeSH terms

  • Biomarkers, Tumor* / blood
  • Biomarkers, Tumor* / genetics
  • Circulating Tumor DNA* / blood
  • Circulating Tumor DNA* / genetics
  • DNA Mutational Analysis
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Liquid Biopsy
  • Mutation*
  • Neoplasms* / blood
  • Neoplasms* / diagnosis
  • Neoplasms* / genetics
  • Pathology, Molecular / standards
  • Surveys and Questionnaires

Substances

  • Circulating Tumor DNA
  • Biomarkers, Tumor