Pathological and genetic characterization of foot and mouth disease viruses collected from cattle and water buffalo in Egypt

PLoS One. 2023 Oct 11;18(10):e0291970. doi: 10.1371/journal.pone.0291970. eCollection 2023.

Abstract

Foot-and-mouth disease (FMD), a highly contagious viral disease caused by FMD virus (FMDV) that threatens Egypt's livestock industry. FMDV causes severe economic losses in the livestock, with restriction of international trade from endemic regions. Surveillance for FMDV serotypes circulating in Egypt is urgently needed to assess the epidemiological situation in the country. FMD outbreaks reported in Egypt in between December 2016 and January-March 2017. A cross-sectional study was conducted to identify the FMDV serotypes responsible for the outbreaks and to collect information on the virus's morphopathological effects. Postmortem tissue and clinical samples (oral swabs, vesicular fluids from ruptured vesicles, and blood) were collected from recently deceased and infected animals. Pathological examination revealed classical FMD lesions as vesicular and erosive lesions on epithelial tissues with non-suppurative lymphoplasmacytic myocarditis. Phylogenetic and sequencing analyses demonstrated that FMDV serotype O, EA-3 topotype, VP1 is the prevalent serotype responsible for the pathological alterations and the high mortality in young calves, adult cattle, and water buffalo. The outcomes indicate continuous mutations in the circulating FMDV, which result in the occasional failure of vaccination. Based on these findings, extensive continuous monitoring and serotyping of the existing circulating FMDV isolates and regular vaccination with reevaluation of the currently used vaccine in Egypt are recommended to prevent the recurrence of such outbreaks.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Buffaloes
  • Cattle
  • Cattle Diseases* / epidemiology
  • Commerce
  • Cross-Sectional Studies
  • Disease Outbreaks / prevention & control
  • Egypt / epidemiology
  • Foot-and-Mouth Disease Virus*
  • Foot-and-Mouth Disease*
  • Internationality
  • Phylogeny
  • Serogroup

Grants and funding

This study was supported by King Khalid University in the form of a grant awarded to MA and HH (RGP2/160/44).