In Vitro Tracking of Sporozoites via Fluorescence Imaging and MRI Using Multifunctional Micelles

ACS Appl Bio Mater. 2023 Dec 18;6(12):5324-5332. doi: 10.1021/acsabm.3c00596. Epub 2023 Dec 1.

Abstract

Early detection could increase the treatment efficiency and prevent the recurrence of malaria disease. To track and detect malarial sporozoites, novel drug delivery systems have been explored for their ability to target these parasites specifically. This study investigates the potential of micelles to track Plasmodium vivax by targeting the Plasmodium vivax hexose transporter using glucose-based interactions. In vitro experiments were conducted using glucose/SPIO/Nile red (targeted) micelles and methoxy/SPIO/Nile red (nontargeted) micelles, revealing that the targeted micelles exhibited stronger fluorescence with the sporozoites and higher relative R2* values compared to the nontargeted micelles. These findings suggest that targeted micelles could be used for the specific detection of Plasmodium sporozoites using fluorescence imaging and MRI techniques, offering a promising approach for efficient malaria parasite detection.

Keywords: MRI and detection; glucose; malaria; targeted micelles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Glucose
  • Magnetic Resonance Imaging / methods
  • Malaria*
  • Micelles*
  • Optical Imaging
  • Sporozoites

Substances

  • Micelles
  • ferric oxide
  • Glucose