Romidepsin and Afatinib Abrogate Jak-Signal Transducer and Activator of Transcription Signaling and Elicit Synergistic Antitumor Effects in Cutaneous T-Cell Lymphoma

J Invest Dermatol. 2024 Jul;144(7):1579-1589.e8. doi: 10.1016/j.jid.2023.12.010. Epub 2024 Jan 14.

Abstract

Cutaneous T-cell lymphomas are mature lymphoid neoplasias resulting from the malignant transformation of skin-resident T-cells. A distinctive clinical feature of cutaneous T-cell lymphomas is their sensitivity to treatment with histone deacetylase inhibitors. However, responses to histone deacetylase inhibitor therapy are universally transient and noncurative, highlighting the need for effective and durable drug combinations. In this study, we demonstrate that the combination of romidepsin, a selective class I histone deacetylase inhibitor, with afatinib, an EGFR family inhibitor, induces strongly synergistic antitumor effects in cutaneous T-cell lymphoma models in vitro and in vivo through abrogation of Jak-signal transducer and activator of transcription signaling. These results support a previously unrecognized potential role for histone deacetylase inhibitor plus afatinib combination in the treatment of cutaneous T-cell lymphomas.

Keywords: Combination therapy; Cutaneous T-cell lymphoma; Preclinical models; Romidepsin.

MeSH terms

  • Afatinib* / pharmacology
  • Animals
  • Antineoplastic Combined Chemotherapy Protocols / pharmacology
  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use
  • Cell Line, Tumor
  • Depsipeptides* / administration & dosage
  • Depsipeptides* / pharmacology
  • Drug Synergism*
  • Histone Deacetylase Inhibitors / pharmacology
  • Histone Deacetylase Inhibitors / therapeutic use
  • Humans
  • Janus Kinases / antagonists & inhibitors
  • Janus Kinases / metabolism
  • Lymphoma, T-Cell, Cutaneous* / drug therapy
  • Lymphoma, T-Cell, Cutaneous* / pathology
  • Mice
  • Signal Transduction* / drug effects
  • Skin Neoplasms* / drug therapy
  • Skin Neoplasms* / pathology
  • Xenograft Model Antitumor Assays

Substances

  • romidepsin
  • Depsipeptides
  • Afatinib
  • Janus Kinases
  • Histone Deacetylase Inhibitors