JNK inhibition enhances cell-cell adhesion impaired by desmoglein 3 gene disruption in keratinocytes

Histochem Cell Biol. 2024 Apr;161(4):345-357. doi: 10.1007/s00418-023-02264-8. Epub 2024 Jan 16.

Abstract

c-Jun NH2-terminal protein kinase (JNK) and p38 are stress-activated mitogen-activated protein kinases (MAPK) that are phosphorylated by various stimuli. It has been reported that the loss of desmoglein (DSG) 3, a desmosomal transmembrane core molecule, in keratinocytes impairs cell-cell adhesion accompanied by p38 MAPK activation. To understand the biological role of DSG3 in desmosomes and its relationship with stress-activated MAPKs, we established DSG3 knockout keratinocytes (KO cells). Wild-type cells showed a linear localization of DSG1 to cell-cell contacts, whereas KO cells showed a remarkable reduction despite the increased protein levels of DSG1. Cell-cell adhesion in KO cells was impaired over time, as demonstrated by dispase-based dissociation assays. The linear localization of DSG1 to cell-cell contacts and the strength of cell-cell adhesion were promoted by the pharmacological inhibition of JNK. Conversely, pharmacological activation of JNK, but not p38 MAPK, in wild-type cells reduced the linear localization of DSG1 in cell-cell contacts. Our data indicate that DSG1 and DSG2 in KO cells cannot compensate for the attenuation of cell-cell adhesion strength caused by DSG3 deficiency and that JNK inhibition restores the strength of cell-cell adhesion by increasing the linear localization of DSG1 in cell-cell contacts in KO cells. Inhibition of JNK signaling may improve cell-cell adhesion in diseases in which DSG3 expression is impaired.

Keywords: Desmoglein; Desmosome; Keratinocyte; Mitogen-activated protein kinase.

MeSH terms

  • Cell Adhesion / genetics
  • Desmoglein 3* / genetics
  • Desmoglein 3* / metabolism
  • Keratinocytes* / metabolism
  • MAP Kinase Signaling System

Substances

  • Desmoglein 3