Impact of air humidity on the tenacity of different agents in bioaerosols

PLoS One. 2024 Jan 26;19(1):e0297193. doi: 10.1371/journal.pone.0297193. eCollection 2024.

Abstract

Despite the variety of pathogens that are transmitted via the airborne route, few data are available on factors that influence the tenacity of airborne pathogens. In order to better understand and thus control airborne infections, knowledge of these factors is important. In this study, three agents, S. aureus, G. stearothermophilus spores and the MS2 bacteriophage, were aerosolized at relative humidities (RH) varying between 30% and 70%. Air samples were then analyzed to determine the concentration of the agents. S. aureus was found to have significantly lower survival rate in the aerosol at RH above 60%. It showed the lowest recovery rates of the three agents, ranging from 0.13% at approximately 70% RH to 4.39% at 30% RH. G. stearothermophilus spores showed the highest tenacity with recovery rates ranging from 41.85% to 61.73% with little effect of RH. For the MS2 bacteriophage, a significantly lower tenacity in the aerosol was observed with a recovery rate of 4.24% for intermediate RH of approximately 50%. The results of this study confirm the significant influence of the RH on the tenacity of airborne microorganisms depending on the specific agent. These data show that the behavior of microorganism in bioaerosols is varies under different environmental conditions.

MeSH terms

  • Aerosols / pharmacology
  • Air Microbiology
  • Humidity
  • Spores, Bacterial*
  • Staphylococcus aureus*

Substances

  • Aerosols

Grants and funding

This research was funded by the Federal Ministry of Education and Research Germany within the project InfectControl 2020_EKOS (grant number 03ZZ0817B). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.