Characterization of human CD4+EOMES+GzmK+ T-cell subsets unveils an uncoupling of suppressive functions from IL-10-producing capacities

Eur J Immunol. 2024 Apr;54(4):e2350675. doi: 10.1002/eji.202350675. Epub 2024 Feb 23.

Abstract

Human CD4+EOMES+ T cells are heterogeneous and contain Th1-cells, Tr1-cells, and CD4+CTL. Tr1- cells and non-classical EOMES+ Th1-cells displayed, respectively, anti- and pro-inflammatory cytokine profiles, but both expressed granzyme-K, produced IFN-γ, and suppressed T-cell proliferation. Diffusion map suggested a progressive CD4+T-cell differentiation from naïve to cytotoxic cells and identified EOMES+Th1-cells as putative Tr1-cell precursors (pre-Tr1).

MeSH terms

  • CD4-Positive T-Lymphocytes
  • Cell Differentiation
  • Humans
  • Interleukin-10*
  • T-Box Domain Proteins / genetics
  • T-Lymphocyte Subsets*
  • T-Lymphocytes, Regulatory
  • Th1 Cells

Substances

  • Interleukin-10
  • EOMES protein, human
  • T-Box Domain Proteins