Sclerostin antibody enhances implant osseointegration in bone with Col1a1 mutation

Bone. 2024 Sep:186:117167. doi: 10.1016/j.bone.2024.117167. Epub 2024 Jun 13.

Abstract

We evaluated the potential of sclerostin antibody (SclAb) therapy to enhance osseointegration of dental and orthopaedic implants in a mouse model (Brtl/+) mimicking moderate to severe Osteogenesis Imperfecta (OI). To address the challenges in achieving stable implant integration in compromised bone conditions, our aim was to determine the effectiveness of sclerostin antibody (SclAb) at improving bone-to-implant contact and implant fixation strength. Utilizing a combination of micro-computed tomography, mechanical push-in testing, immunohistochemistry, and Western blot analysis, we observed that SclAb treatment significantly enhances bone volume fraction (BV/TV) and bone-implant contact (BIC) in Brtl/+ mice, suggesting a normalization of bone structure toward WT levels. Despite variations in implant survival rates between the maxilla and tibia, SclAb treatment consistently improved implant stability and resistance to mechanical forces, highlighting its potential to overcome the inherent challenges of OI in dental and orthopaedic implant integration. These results suggest that SclAb could be a valuable therapeutic approach for enhancing implant success in compromised bone conditions.

Keywords: Alveolar bone; Maxilla; Osteogenesis imperfecta; Sclerostin; Tibia.

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Animals
  • Antibodies* / pharmacology
  • Bone and Bones / diagnostic imaging
  • Bone and Bones / drug effects
  • Bone and Bones / pathology
  • Collagen Type I* / genetics
  • Collagen Type I* / metabolism
  • Dental Implants
  • Intercellular Signaling Peptides and Proteins
  • Mice
  • Mutation* / genetics
  • Osseointegration* / drug effects
  • Tibia / diagnostic imaging
  • Tibia / drug effects
  • Tibia / pathology
  • X-Ray Microtomography

Substances

  • Sost protein, mouse
  • Adaptor Proteins, Signal Transducing
  • Collagen Type I
  • Antibodies
  • Intercellular Signaling Peptides and Proteins
  • Dental Implants