Comparative physiological and transcriptomic analyses provide induction resistance mechanisms of Bacillus tequilensis against Colletotrichum fructicola in Camellia oleifera

Aiting Zhou; Junrong Tang; Qianjie Du; Jia Deng; Jianrong Wu; Huancheng Ma; Fang Wang

doi:10.1016/j.plaphy.2024.108912

Comparative physiological and transcriptomic analyses provide induction resistance mechanisms of Bacillus tequilensis against Colletotrichum fructicola in Camellia oleifera

Plant Physiol Biochem. 2024 Sep:214:108912. doi: 10.1016/j.plaphy.2024.108912. Epub 2024 Jul 4.

Authors

Aiting Zhou¹, Junrong Tang¹, Qianjie Du¹, Jia Deng², Jianrong Wu³, Huancheng Ma⁴, Fang Wang⁵

Affiliations

¹ Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming, 650224, PR China.
² Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, 650224, PR China.
³ Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming, 650224, PR China; Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province, Southwest Forestry University, Kunming, 650224, PR China.
⁴ Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming, 650224, PR China. Electronic address: [email protected].
⁵ Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming, 650224, PR China; Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, 650224, PR China. Electronic address: [email protected].

PMID: 38972241
DOI: 10.1016/j.plaphy.2024.108912

Abstract

Bacillus tequilensis DZY 6715 was isolated from healthy leaves in Camellia oleifera, and the strain DZY 6715 significantly inhibited anthracnose disease resulting from Colletotrichum fructicola in C. oleifera, besides, its associated mechanism of disease resistance was explored. B. tequilensis DZY 6715 treatment controlled mycelial growth of C. fructicola in C. oleifera, and significantly decreased C. oleifera anthracnose incidence and disease index compared with the control group. B. tequilensis DZY 6715 has strong biofilm forming ability, and also secretes extracellular β-1, 3-glucanase and chitinase, which could cause cell membranes damage and increased cellular compound leakage. C.oleifera treated with DZY 6715 also effectively enhanced enzyme activities and stimulated the synthesis the substances related to phenylpropane metabolism and reactive oxygen metabolism. Moreover, transcript profiling analysis revealed more differentially expressed genes related to phenylpropanoid pathway metabolism and antioxidant system inducing by DZY 6715 compared with the control in C. oleifera. Thus, it can be concluded that B. tequilensis DZY 6715 is a suitable bio-control agent to control anthracnose disease in C. oleifera.

Keywords: Anthracnose; Bacillus tequilensis; Biological control; Camellia oleifera.

Publication types

Comparative Study

MeSH terms

Bacillus* / genetics
Bacillus* / physiology
Camellia* / genetics
Camellia* / microbiology
Colletotrichum* / physiology
Disease Resistance / genetics
Gene Expression Profiling
Plant Diseases* / microbiology
Transcriptome