Methylglyoxal-derived hydroimidazolone-1/RAGE axis induces renal oxidative stress and renal fibrosis in vitro and in vivo

Toxicology. 2024 Sep:507:153887. doi: 10.1016/j.tox.2024.153887. Epub 2024 Jul 15.

Abstract

Advanced glycation end products (AGEs) are important contributors to the progression of chronic kidney diseases (CKD), including renal fibrosis. Although the relationship between AGEs and renal fibrosis has been well studied, the mechanisms of individual AGE-induced renal injury remain poorly understood. This study investigated the adverse effect of methylglyoxal-derived hydroimidazolone-1 (MG-H1), a methylglyoxal (MG)-derived AGE generated by the glycation of MG and arginine residues, on kidney damage. We aimed to elucidate the molecular mechanisms of MG-H1-mediated renal injury and fibrosis, focusing on the receptor for AGEs (RAGE) signaling and its effects on the Wnt/β-catenin pathway, MAPK pathway, and inflammatory responses. Our results suggest that the MG-H1/RAGE axis plays a significant role in the pathogenesis of CKD and its downstream events involving MAPK kinase-related factors and inflammatory factors. MG-H1 treatment modulated the expression of inflammatory cytokines (TNF-α, IL-6, and IL-1β) and MAPK proteins (ERK1/2, JNK, and p38).

Keywords: Advanced glycation end products; Chronic kidney diseases; Fibrosis; Kidney; Methylglyoxal-derived hydroimidazolone-1.

MeSH terms

  • Animals
  • Cytokines / metabolism
  • Fibrosis*
  • Glycation End Products, Advanced / metabolism
  • Glycation End Products, Advanced / toxicity
  • Humans
  • Imidazoles* / pharmacology
  • Imidazoles* / toxicity
  • Kidney* / drug effects
  • Kidney* / metabolism
  • Kidney* / pathology
  • MAP Kinase Signaling System / drug effects
  • Male
  • Mice
  • Oxidative Stress* / drug effects
  • Pyruvaldehyde* / toxicity
  • Receptor for Advanced Glycation End Products* / metabolism
  • Renal Insufficiency, Chronic / chemically induced
  • Renal Insufficiency, Chronic / metabolism
  • Renal Insufficiency, Chronic / pathology

Substances

  • Pyruvaldehyde
  • Imidazoles
  • Receptor for Advanced Glycation End Products
  • Cytokines
  • Glycation End Products, Advanced