Six novel species of the genus Methanoculleus isolated from various environments in Taiwan

Shu-Jung Lai; Mei-Chin Lai; Saulwood Lin; Yi-Ting You; Wen-Hsin Wei; Yu-Wei Chen; Zih-Han Lan; Chia-Hsiu Fan; Sue-Yao Wu; Chuan-Chuan Hung; Jiun-Yan Ding; Wei-Ling Zhang; Yu-Chen Deng; Yu-Chien Lee; Chao-Jen Shih; Yen-Chi Wu; Jingjing Zhao; Yin Li; Sheng-Chung Chen

doi:10.1099/ijsem.0.006477

Six novel species of the genus Methanoculleus isolated from various environments in Taiwan

Int J Syst Evol Microbiol. 2024 Jul;74(7). doi: 10.1099/ijsem.0.006477.

Authors

Shu-Jung Lai^{1

2

3}, Mei-Chin Lai¹, Saulwood Lin⁴, Yi-Ting You¹, Wen-Hsin Wei¹, Yu-Wei Chen¹, Zih-Han Lan¹, Chia-Hsiu Fan¹, Sue-Yao Wu¹, Chuan-Chuan Hung¹, Jiun-Yan Ding¹, Wei-Ling Zhang^{1

5}, Yu-Chen Deng¹, Yu-Chien Lee¹, Chao-Jen Shih⁵, Yen-Chi Wu⁵, Jingjing Zhao^{6

7}, Yin Li^{6

7

8

9}, Sheng-Chung Chen^{1

6

7

8}

Affiliations

¹ Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan, ROC.
² Graduate Institute of Biomedical Sciences, China Medical University, Taichung City, Taiwan, ROC.
³ Research Center for Cancer Biology, China Medical University, Taichung City, Taiwan, ROC.
⁴ Institute of Oceanography, National Taiwan University, Taipei, Taiwan, ROC.
⁵ Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan, ROC.
⁶ School of Resources and Chemical Engineering, Sanming University, Sanming, Fujian, PR China.
⁷ Fujian Provincial Key Laboratory of Resources and Environmental Monitoring and Sustainable Management and Utilization, Sanming University, Sanming, Fujian, PR China.
⁸ College of Environment and Safety Engineering, Fuzhou University, Fuzhou, Fujian, PR China.
⁹ Medical Plant Exploitation and Utilization Engineering Research Center, Sanming University, Sanming, Fujian, PR China.

PMID: 39083039
DOI: 10.1099/ijsem.0.006477

Abstract

Taiwan is situated in the subtropical region and its geographical location and topographical features contribute to a rich ecological diversity and scenic landscapes. We investigated the diversity of methanogens in different environments of Taiwan using a culture-dependent method. This report presents the characterization and taxonomy of six hydrogenotrophic methanogens obtained from cold seep sediments (strain FWC-SCC1^T and FWC-SCC3^T), marine sediments (strain CWC-02^T and YWC-01^T), estuarine sediments (strain Afa-1^T), and a hot spring well (strain Wushi-C6^T) in Taiwan. The proposed names of the six novel species are Methanoculleus frigidifontis (type strain FWC-SCC1^T=BCRC AR10056^T=NBRC 113993^T), Methanoculleus oceani (CWC-02^T=BCRC AR10055^T=NBRC 113992^T), Methanoculleus methanifontis (FWC-SCC3^T=BCRC AR10057^T=NBRC 113994^T), Methanoculleus nereidis (YWC-01^T=BCRC AR10060^T=NBRC 114597^T), Methanoculleus formosensis (Afa-1^T=BCRC AR10054^T=NBRC 113995^T), and Methanoculleus caldifontis (Wushi-06^T=BCRC AR10059^T= NBRC 114596^T).

Keywords: Marine sediment; Methanoculleus; cold seep; hot spring; methane hydrate; methane seep.

MeSH terms

Base Composition
DNA, Archaeal* / genetics
Geologic Sediments* / microbiology
Hot Springs / microbiology
Methanomicrobiaceae / classification
Methanomicrobiaceae / genetics
Methanomicrobiaceae / isolation & purification
Phylogeny*
RNA, Ribosomal, 16S* / genetics
Sequence Analysis, DNA*
Taiwan

Substances

RNA, Ribosomal, 16S
DNA, Archaeal