Targeted mutagenesis in Arabidopsis and medicinal plants using transposon-associated TnpB

Zongyou Lv; Wenhua Chen; Shiyuan Fang; Boran Dong; Xingxing Wang; Lida Zhang; Jingshi Xue; Wansheng Chen

doi:10.1111/jipb.13758

Targeted mutagenesis in Arabidopsis and medicinal plants using transposon-associated TnpB

J Integr Plant Biol. 2024 Oct;66(10):2083-2086. doi: 10.1111/jipb.13758. Epub 2024 Aug 7.

Authors

Zongyou Lv¹, Wenhua Chen¹, Shiyuan Fang¹, Boran Dong¹, Xingxing Wang¹, Lida Zhang², Jingshi Xue³, Wansheng Chen^{1

4}

Affiliations

¹ Research and Development Center of Chinese Medicine Resources and Biotechnology, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
² Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
³ Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 201203, China.
⁴ Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China.

PMID: 39109980
DOI: 10.1111/jipb.13758

Abstract

The programmable nuclease TnpB is significantly smaller than Cas9, can edit genes in medicinal plants, including Artemisia annua, Salvia miltiorrhiza, Scutellaria baicalensis, Isatis indigotica, and Codonopsis pilosula, and has potential uses in molecular breeding to enhance crop yield and quality.

Targeted mutagenesis in Arabidopsis and medicinal plants using transposon-associated TnpB

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