Lipid Composition, Cytotoxic and Acetylcholinesterase Inhibition Effects of Two Brown Algae Species Lobophora tsengii and Lobophora australis

J Oleo Sci. 2024;73(9):1177-1187. doi: 10.5650/jos.ess24002.

Abstract

In this paper, the lipid classes, compositions of the neutral lipids, phospholipids and fatty acids, acetylcholinesterase inhibition and cytotoxic activity of two brown algae Lobophora tsengii D. Tien & Z. Sun and Lobophora australis Z. Sun, F. C. Gurgel & H. Kawai have been investigated. The polar lipid class had the highest content in total lipid (TL) (43.47% in L. tsengii and 48.95% in L. australis). Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were the main components in the phospholipids of two studied brown algae with contents varied from 32.27% to 52.33%. Total lipids were rich in PUFA (42.54% of total fatty acids for L. australis and 32.98% for L. tsengii), with EPA (11.46%, 14.30%) and AA (8.0%, 11.96%). L. tsengii methanol extract inhibited acetylcholinesterase (AChE) in in vitro assay with an IC50 value of 25.45 µg/mL. Both Lobophora methanol extracts display cytotoxic effects against four human cancer cell lines (KB, MCF7, HepG2 and A549) with IC50 in the range of 21.11-83.61 µg/mL. Especially, L. australis extract showed a strong cytotoxicity against KB cell lines with IC50 value of 21.11±0.39 µg/mL.

Keywords: Lobophora australis; Lobophora tsengii; acetylcholinesterase; cytotoxicity; fatty acid; phospholipid class.

MeSH terms

  • Acetylcholinesterase* / metabolism
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology
  • Cell Line, Tumor
  • Cholinesterase Inhibitors* / isolation & purification
  • Cholinesterase Inhibitors* / pharmacology
  • Fatty Acids / analysis
  • Fatty Acids, Unsaturated / pharmacology
  • Humans
  • Lipids
  • Phaeophyceae* / chemistry
  • Phosphatidylcholines
  • Phosphatidylethanolamines
  • Phospholipids

Substances

  • Cholinesterase Inhibitors
  • Acetylcholinesterase
  • Phospholipids
  • Lipids
  • Fatty Acids
  • Phosphatidylethanolamines
  • Phosphatidylcholines
  • phosphatidylethanolamine
  • Antineoplastic Agents
  • Fatty Acids, Unsaturated