Trabecular Meshwork Regeneration for Glaucoma Treatment Using Stem Cell-Derived Trophic Factors

Ajay Kumar; Enzhi Yang; Yiqin Du

doi:10.1007/978-1-0716-4087-6_4

Trabecular Meshwork Regeneration for Glaucoma Treatment Using Stem Cell-Derived Trophic Factors

Methods Mol Biol. 2025:2848:59-71. doi: 10.1007/978-1-0716-4087-6_4.

Authors

Ajay Kumar¹, Enzhi Yang², Yiqin Du³

Affiliations

¹ Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.
² Department of Ophthalmology, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
³ Department of Ophthalmology, Morsani College of Medicine, University of South Florida, Tampa, FL, USA. [email protected].

PMID: 39240516
DOI: 10.1007/978-1-0716-4087-6_4

Abstract

Glaucoma is one of the leading causes of irreversible blindness. Stem cell therapy has shown promise in the treatment of primary open-angle glaucoma in animal models. Stem cell-free therapy using stem cell-derived trophic factors might be in demand in patients with high-risk conditions or religious restrictions. In this chapter, we describe methods for trabecular meshwork stem cell (TMSC) cultivation, secretome harvesting, and protein isolation, as well as assays to ensure the health of TMSC post-secretome harvesting and for secretome periocular injection into mice for therapeutic purposes.

Keywords: Glaucoma; Periocular injection; Secretome; Stem cells; Trabecular meshwork.

MeSH terms

Animals
Cell Culture Techniques / methods
Cells, Cultured
Disease Models, Animal
Glaucoma / therapy
Glaucoma, Open-Angle / therapy
Humans
Intercellular Signaling Peptides and Proteins / metabolism
Intercellular Signaling Peptides and Proteins / pharmacology
Mice
Regeneration
Secretome
Stem Cell Transplantation / methods
Stem Cells* / cytology
Stem Cells* / metabolism
Trabecular Meshwork* / cytology
Trabecular Meshwork* / metabolism

Substances

Intercellular Signaling Peptides and Proteins