Modified smear method for screening potential inhibitors of platelet aggregation from plant sources

J Nat Prod. 1985 May-Jun;48(3):363-70. doi: 10.1021/np50039a003.

Abstract

The smear method developed by Velaskar and Chitre was modified to allow the massive screening of plant extracts and/or fractions for platelet antiaggregating activity. Aspirin and papaverine, known inhibitors of platelet aggregation, were tested by the modified method and showed comparable results with previously reported data. Fifty-four fractions prepared from eighteen plant species were screened for their inhibitory effects on adenosine 5'-diphosphate, arachidonic acid, or collagen-induced rat platelet aggregation. The results suggest that plants could be a potential source of inhibitors of platelet aggregation.

MeSH terms

  • Adenosine Diphosphate / pharmacology
  • Animals
  • Arachidonic Acid
  • Arachidonic Acids / pharmacology
  • Aspirin / pharmacology
  • Collagen / pharmacology
  • Drug Evaluation, Preclinical
  • In Vitro Techniques
  • Male
  • Papaverine / pharmacology
  • Plant Extracts / pharmacology*
  • Platelet Aggregation / drug effects*
  • Rats
  • Rats, Inbred Strains

Substances

  • Arachidonic Acids
  • Plant Extracts
  • Arachidonic Acid
  • Adenosine Diphosphate
  • Collagen
  • Papaverine
  • Aspirin