β-Lactoglobulin Enhances Clay and Activated Carbon Binding and Protection Properties for Cadmium and Lead

Kendall Lilly; Meichen Wang; Asuka A Orr; Sarah E Bondos; Timothy D Phillips; Phanourios Tamamis

doi:10.1021/acs.iecr.4c01774

β-Lactoglobulin Enhances Clay and Activated Carbon Binding and Protection Properties for Cadmium and Lead

Ind Eng Chem Res. 2024 Sep 6;63(37):16124-16140. doi: 10.1021/acs.iecr.4c01774. eCollection 2024 Sep 18.

Authors

Kendall Lilly¹, Meichen Wang^{2

3

4}, Asuka A Orr⁵, Sarah E Bondos⁶, Timothy D Phillips^{2

3}, Phanourios Tamamis^{1

5}

Affiliations

¹ Department of Materials Science and Engineering, College of Engineering, Texas A&M University, College Station, Texas 77843, United States.
² Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843, United States.
³ Interdisciplinary Faculty of Toxicology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843, United States.
⁴ Department of Environmental Health Sciences, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States.
⁵ Artie McFerrin Department of Chemical Engineering, College of Engineering, Texas A&M University, College Station, Texas 77843, United States.
⁶ Department of Medical Physiology Texas A&M Health Science Center, Texas A&M University, College Station, Texas 77843, United States.

Abstract

The removal of heavy metals from wastewater remains a challenge due to the limitations of current remediation methods. This study aims to develop multicomponent composites as inexpensive and environmentally friendly sorbents with enhanced capture of cadmium (Cd) and lead (Pb). The composites are based on calcium montmorillonite (CM) and activated carbon (AC) because of their proven effectiveness as sorbents for diverse toxins in environmental settings. In this study, we used a combination of computational and experimental methods to delineate that β-lactoglobulin enhances CM and AC binding and protection properties for Cd and Pb. Modeling and molecular dynamics simulations investigated the formation of material systems formed by CM and AC in complex with β-lactoglobulin and predicted their capacity to bind heavy metal ions at neutral pH conditions. Our simulations suggest that the enhanced binding properties of the material systems are attributed to the presence of several binding pockets formed by β-lactoglobulin for the two heavy metal ions. At neutral pH conditions, divalent Cd and Pb shared comparable binding propensities in all material systems, with the former being consistently higher than the latter. To validate the interactions depicted in simulations, two ecotoxicological models (L. minor and H. vulgaris) were exposed to Cd, Pb, and a mixture of the two. The inclusion of CM-lactoglobulin (β-lactoglobulin amended CM) and AC-lactoglobulin (β-lactoglobulin amended AC) at 0.05-0.2% efficiently and dose-dependently reduced the severe toxicity of metals and increased the growth parameters. This high efficacy of protection shown in the ecotoxicological models may result from the numerous possible interaction pockets of the β-lactoglobulin-amended materials depicted in simulations. The ecotoxicological models support the agreement with computations. This study serves as a proof of concept on how computations in tandem with experiments can be used in the design of multicomponent clay- and carbon-based sorbent amended systems with augmented functionalities for particular toxins.