Bioremediation of azo dye: A review on strategies, toxicity assessment, mechanisms, bottlenecks and prospects

The synthetic azo dyes are widely used in the textile industries for their excellent dyeing properties. They may be classified into many classes based on their structure and application, including direct, reactive, dispersive, acidic, basic, and others. The continuous discharge of wastewater from a large number of textile industries without prior treatment poses detrimental effects on the environment and human health. Azo dyes and their degradation products are extremely poisonous for their carcinogenic, teratogenic and mutagenic nature. Moreover, exposure to synthetic azo dyes can cause genetic changes, skin inflammation, hypersensitivity responses, and skin irritations in persons, which may ultimately result in other profound issues including the deterioration of water quality. This review discusses these dyes in details along with their detrimental effects on aquatic and terrestrial flora and fauna including human beings. Azo dyes degrade the water bodies by increasing biochemical and chemical oxygen demand. Therefore, dye-containing wastewater should be effectively treated using eco-friendly and cost-effective technologies to avoid negative impact on the environment. This article extensively reviews on physical, chemical and biological treatment with their benefits and challenges. Biological-based treatment with higher hydraulic retention time (HRT) is economical, consumes less energy, produces less sludge and environmentally friendly. Whereas the physical and chemical methods with less hydraulic retention time is costly, produces large sludge, requires high dissolved oxygen and ecologically inefficient. Since, biological treatment is more advantageous over physical and chemical methods, researchers are concentrating on bioremediation for eliminating harmful azo dye pollutants from nature. This article provides a thorough analysis of the state-of-the-art biological treatment technologies with their developments and effectiveness in the removal of azo dyes. The mechanism by which genes encoding azoreductase enzymes (azoG, and azoK) enable the natural degradation of azo dyes by bacteria and convert them into less harmful compounds is also extensively examined. Therefore, this review also focuses on the use of genetically modified microorganisms and nano-technological approaches for bioremediation of azo dyes.