SWATH-MS Based Secretome Proteomic Analysis of Pseudomonas aeruginosa Against MRSA

Yi-Feng Zheng; Yu-Sheng Lin; Jing-Wen Huang; Kuo-Tung Tang; Cheng-Yu Kuo; Wei-Chen Wang; Han-Ju Chien; Chih-Jui Chang; Nien-Jen Hu; Chien-Chen Lai

doi:10.1002/pmic.202300649

SWATH-MS Based Secretome Proteomic Analysis of Pseudomonas aeruginosa Against MRSA

Proteomics. 2024 Oct 17:e202300649. doi: 10.1002/pmic.202300649. Online ahead of print.

Authors

Yi-Feng Zheng¹, Yu-Sheng Lin^{1

2}, Jing-Wen Huang¹, Kuo-Tung Tang^{3

4}, Cheng-Yu Kuo¹, Wei-Chen Wang¹, Han-Ju Chien⁵, Chih-Jui Chang⁶, Nien-Jen Hu⁷, Chien-Chen Lai^{1

4

8

9

10}

Affiliations

¹ Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan.
² Department of Internal Medicine, Division of Chest Medicine, Asia University Hospital, Asia University, Taichung, Taiwan.
³ Division of Allergy, Immunology and Rheumatology, Taichung Veterans General Hospital, Taichung, Taiwan.
⁴ Doctoral Program in Translational Medicine, National Chung Hsing University, Taichung, Taiwan.
⁵ Department of Biochemical Science and Technology, National Chiayi University, Chiayi, Taiwan.
⁶ Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien, Taiwan.
⁷ Institute of Biochemistry, National Chung Hsing University, Taichung, Taiwan.
⁸ Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung, Taiwan.
⁹ Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan.
¹⁰ Rong Hsing Translational Medicine Research Center, National Chung Hsing University, Taichung, Taiwan.

PMID: 39420696
DOI: 10.1002/pmic.202300649

Abstract

The study uses Sequential Window Acquisition of All Theoretical Fragment Ion Mass Spectra (SWATH)-MS in conjunction with secretome proteomics to identify key proteins that Pseudomonas aeruginosa secretes against methicillin-resistant Staphylococcus aureus (MRSA). Variations in the inhibition zones indicated differences in strain resistance. Multivariate statistical methods were applied to filter the proteomic results, revealing five potential protein biomarkers, including Peptidase M23. Gene ontology (GO) analysis and sequence alignment supported their antibacterial activity. Thus, SWATH-MS provides a comprehensive understanding of the secretome of P. aeruginosa in its action against MRSA, guiding future antibacterial research.

Keywords: Pseudomonas aeruginosa; SWATH‐MS; methicillin‐resistant Staphylococcus aureus; proteomics; scretome.

Abstract

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