Identification of the 4CL family in cassava (Manihot esculenta Crantz) and expression pattern analysis of the Me4CL32 gene

The 4-coumarate coenzyme A ligase (4CL) plays a critical role in the phenylpropane metabolic pathway and is a key enzyme in plant growth metabolism and stress responses. Using bioinformatics methods, 50 Me4CL gene were identified within the cassava genome u, and a comprehensive analysis of the cassava 4CL gene family was conducted. The results showed that these 50 4CL proteins are divided into four subfamilies, with members within the same subfamily sharing similar or identical gene structures. Co-linearity analysis revealed that cassava and rubber trees have the highest number of homologous genes, indicating a close homologous relationship between them. Analysis of 20 cis-acting elements in the promoter region of Me4CL32 revealed the presence of hormone-responsive elements such as gibberellin, auxin, abscisic acid, and as well as elements related to meristematic tissue regulation. results Quantitative real-time PCR (qRT-PCR) results showed that Me4CL32 gene expression changes in response to abiotic stressors (drought, salt, cold, heat) and hormonal stimuli(GA3 and ABA), indicating that Me4CL32 can respond to both environmental pressures and hormone signals. RNA-seq transcriptome and single-cell transcriptome analyses were used to examine the expression patterns of Me4CLs. Additionally, subcellular localization studies demonstrated that the Me4CL32 protein is confined to the chloroplasts of cassava leaves.Investigating the functionality of this gene family aids in comprehending plant growth, development, and stress resistance mechanisms. Furthermore, it furnishes a theoretical basis for future research on developing resilient cassava germplasm and the enhancing cassava's environmental tolerance.