Detection of Circulating Tumor DNA in Lymphoma Patients

Methods Mol Biol. 2025:2865:475-490. doi: 10.1007/978-1-0716-4188-0_21.

Abstract

Liquid biopsy has become an opportunity in lymphoma diagnostics, since plasma circulating tumor DNA (ctDNA) is an easily accessible source of tumor DNA, which can be complementary to tissue biopsy. Through ctDNA, lymphomas can be molecularly characterized, tumor clonal evolution can be tracked, while monitoring minimal residual diseases during and after therapy. Here, we describe the methodology of cancer personalized profiling by deep sequencing (CAPP-seq) that we use for ctDNA qualification and quantification.

Keywords: CAPP-seq; Liquid biopsy; Lymphoma; Next-generation sequencing; Somatic mutations; cfDNA.

MeSH terms

  • Biomarkers, Tumor* / blood
  • Biomarkers, Tumor* / genetics
  • Circulating Tumor DNA* / blood
  • Circulating Tumor DNA* / genetics
  • High-Throughput Nucleotide Sequencing* / methods
  • Humans
  • Liquid Biopsy / methods
  • Lymphoma* / blood
  • Lymphoma* / diagnosis
  • Lymphoma* / genetics
  • Precision Medicine / methods

Substances

  • Circulating Tumor DNA
  • Biomarkers, Tumor