Synergistic effects of plastic debris and elevated nitrate concentrations on the proliferation of Ulva lactuca micro-propagules

Ulva lactuca is a versatile intertidal and blooming green macroalga with a global distribution, undergoing a lifecycle that consists of critical phases. The effects of surface physical properties and elevated nitrate concentrations on the growth of Ulva spores were evaluated using ATR-FTIR analysis, water contact angle measurements and fluorescence microscopy. The weathering process conducted with seawater led to the emergence of new functional groups such as CO, CH₃ (rock), and CH, alongside a reduction in existing groups on the polymers, as evidenced by ATR-FTIR. This resulted in a progressive increase in surface wettability, with contact angles decreasing on all substrates, particularly in PP which showed the highest contact angle, dropping from 94.8° ± 0.02 to 70.5° ± 0.03. It reflected on the rate of attachment of spores on each plastic. Moreover, the nitrate concentration collectively controlled the density and development of all substrates. Growth rate after 5 days of attachment, most notably on day 10 (N3 60 μM NO₃: length 36.4 ± 2.495 µm) and day 20 (N3 60 μM NO₃: length 181 ± 8.335 µm) increased drastically. Meanwhile, the density was altered in a decreased way throughout the experiment based on substrates. Spore density demonstrated a complex interaction among nitrate and plastics relative to length at all the periods of analysis. These findings can contribute to understanding the role of plastics and Ulva in the ecological phenomena. Consequently, increasing amounts of plastic debris and excessive load of nitrate effluents in marine environments could lead to more frequent algal blooms.