Characterization of Collaborative Cross mouse founder strain CAST/EiJ as a novel model for lethal COVID-19

Sci Rep. 2024 Oct 24;14(1):25147. doi: 10.1038/s41598-024-77087-1.

Abstract

Mutations in SARS-CoV-2 variants of concern (VOCs) have expanded the viral host range beyond primates, and a few other mammals, to mice, affording the opportunity to exploit genetically diverse mouse panels to model the broad spectrum of responses to infection in patient populations. Here we surveyed responses to VOC infection in genetically diverse Collaborative Cross (CC) founder strains. Infection of wild-derived CC founder strains produced a broad range of viral burden, disease susceptibility and survival, whereas most other strains were resistant to disease despite measurable lung viral titers. In particular, CAST/EiJ, a wild-derived strain, developed high lung viral burdens, more severe lung pathology than seen in other CC strains, and a dysregulated cytokine profile resulting in morbidity and mortality. These inbred mouse strains may serve as a valuable platform to evaluate therapeutic countermeasures against severe COVID-19 and other coronavirus pandemics in the future.

MeSH terms

  • Animals
  • COVID-19* / mortality
  • COVID-19* / virology
  • Collaborative Cross Mice / genetics
  • Cytokines / metabolism
  • Disease Models, Animal*
  • Female
  • Humans
  • Lung / pathology
  • Lung / virology
  • Male
  • Mice
  • SARS-CoV-2* / genetics
  • SARS-CoV-2* / isolation & purification
  • SARS-CoV-2* / pathogenicity
  • Viral Load

Substances

  • Cytokines

Supplementary concepts

  • SARS-CoV-2 variants