Molecular-level insights into the leachates released from ultraviolet-aged biodegradable and conventional commercial microplastics and their mechanism of toxicity toward Chlorella pyrenoidosa

Understanding the harmful effects of microplastics (MPs) and their derivatives is a priority in environmental study. However, the characteristics and toxic effects of leachates from MPs at the molecular-level remain unclear. Herein, two conventional commercial MPs [polystyrene (PS) and polyethylene (PE)] and two biodegradable commercial MPs [polylactic acid (PLA) and polybutylene adipate-co-terephthalate/PLA (PBAT/PLA)] were subjected to leaching under ultraviolet-irradiation, and their leachates were investigated. The results showed that the surface morphology of MPs increased in roughness after ultraviolet-irradiation treatment, especially for biodegradable MPs, meanwhile, the particle size of four MPs decreased in various degrees. The biodegradable MPs released several times more dissolved organic matter (DOM) and nano-plastic particles than conventional MPs. Fourier transform ion cyclotron resonance mass spectrometry revealed that lignin-like substances were the predominant component of MP-DOM, followed by protein- and tannin-like substances. The molecular composition and characteristics of the DOM varied significantly among MPs. Transcriptomic analysis showed that 737 and 1259 genes, respectively, were differentially expressed in Chlorella pyrenoidosa in PLA- and PBAT/PLA-MP leachate-treated groups compared with controls, more than in the PS (352) and PE (355) groups. These findings, verified by physiological and histopathological analyses, indicate that the leachates from the biodegradable MPs induced more damage to Chlorella pyrenoidosa than those from the conventional MPs. This is mainly attributed to far more DOM and nano-plastic particles containing in leachates of biodegradable MPs than these of conventional MPs. This study deepens our comprehension of the potential hazards of MP-leachates, and promotes the prudent use and disposal of plastic products.