Transcriptome Analysis of Chinese Cabbage Infected with Plasmodiophora Brassicae in the Primary Stage

Sci Rep. 2024 Oct 30;14(1):26180. doi: 10.1038/s41598-024-76634-0.

Abstract

Clubroot disease caused by the infection of Plasmodiophora brassicae is widespread in China, and significantly reduces the yield of Chinese cabbage (Brassica rapa L. ssp. pekinensis). However, the resistance mechanism of Chinese cabbage against clubroot disease is still unclear. It is important to exploit the key genes that response to early infection of P. brassicae. In this study, it was found that zoospores were firstly invaded hair roots on the 8th day after inoculating with 1 × 107 spores/mL P. brassicae. Transcriptome analysis found that the early interaction between Chinese cabbage and P. brassicae caused the significant expression change of some defense genes, such as NBS-LRRs and pathogenesis-related genes, etc. The above results were verified by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Otherwise, peroxidase (POD) salicylic acid (SA) and jasmonic acid (JA) were also found to be important signal molecules in the resistance to clubroot disease in Chinese cabbage. This study provides important clues for understanding the resistance mechanism of Chinese cabbage against clubroot disease.

MeSH terms

  • Brassica rapa / genetics
  • Brassica rapa / parasitology
  • Brassica* / genetics
  • Brassica* / parasitology
  • Cyclopentanes / metabolism
  • Disease Resistance / genetics
  • Gene Expression Profiling*
  • Gene Expression Regulation, Plant*
  • Oxylipins / metabolism
  • Plant Diseases* / genetics
  • Plant Diseases* / parasitology
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plant Roots / genetics
  • Plant Roots / parasitology
  • Plasmodiophorida* / physiology
  • Salicylic Acid / metabolism
  • Transcriptome

Substances

  • Salicylic Acid
  • jasmonic acid
  • Oxylipins
  • Cyclopentanes
  • Plant Proteins