Recurrence patterns and evolution of submicroscopic and asymptomatic Plasmodium vivax infections in malaria-endemic areas of the Peruvian Amazon

PLoS Negl Trop Dis. 2024 Oct 31;18(10):e0012566. doi: 10.1371/journal.pntd.0012566. eCollection 2024 Oct.

Abstract

Background: In the Peruvian Amazon, Plasmodium vivax malaria transmission is maintained due to the high frequency of recurrences. By understanding the recurrence rates of submicroscopic and asymptomatic cases, we can develop informed strategies to prevent transmission more efficiently and disrupt the silent transmission cycle.

Methods: A three-year, population-based cohort study was conducted in two sites, Cahuide and Lupuna, within the Loreto region in Peru from 2013 to 2015. The study included 385 individuals and aimed to examine the temporal dynamics of malaria recurrences and their impact on transmission and control.

Results: Individuals from Lupuna presented a higher risk of P. vivax infections compared to Cahuide, where most recurrences were asymptomatic and submicroscopic. It is estimated that a great proportion of these recurrences were due to relapses in both communities. The application of molecular diagnostic method proved to be significantly more effective, detecting 2.3 times more episodes during the follow-up (PCR, 1068; microscopy, 467). PCR identified recurrences significantly earlier, at 151 days after an initial infection, compared to microscopy, which detected them on average after 365 days. Community, occupation and previous malaria infections were factors associated with recurrences. Finally, potential infection evolution scenarios were described where one frequent scenario involved the transition from symptomatic to asymptomatic infections with a mean evolution time of 240 days.

Conclusions: This study explores the contrast in malaria recurrence risk among individuals from two endemic settings, a consequence of prolonged exposure to the parasite. Through the analysis of the evolution scenarios of P. vivax recurrences, it is possible to have a more complete vision of how the transmission pattern changes over time and is conditioned by different factors.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Asymptomatic Infections / epidemiology
  • Child
  • Child, Preschool
  • Cohort Studies
  • Endemic Diseases
  • Female
  • Humans
  • Malaria, Vivax* / epidemiology
  • Malaria, Vivax* / parasitology
  • Malaria, Vivax* / transmission
  • Male
  • Microscopy
  • Middle Aged
  • Peru / epidemiology
  • Plasmodium vivax* / genetics
  • Plasmodium vivax* / isolation & purification
  • Recurrence*
  • Young Adult

Grants and funding

This research was supported by the International Center of Excellence in Malaria Research (ICEMR), awarded to JMV in collaboration with DG and MF, under the agreement U19AI089681 from the US Public Health Service, National Institutes of Health/National Institute of Allergy and Infectious Diseases (NIH/NIAID), USA. Additional support was provided by the Fogarty Training Grant (2D43TW007120-11A1, NIH-USA), awarded to JMV and DG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.