TME-Activated MnO2/Pt Nanoplatform of Hydroxyl Radical and Oxygen Generation to Synergistically Promote Radiotherapy and MR Imaging of Glioblastoma

Int J Nanomedicine. 2024 Nov 1:19:11055-11070. doi: 10.2147/IJN.S474098. eCollection 2024.

Abstract

Purpose: Radiotherapy (RT) is currently recognized as an important treatment for glioblastoma (GBM), however, it is associated with several challenges. One of these challenges is the radioresistance caused by hypoxia, whereas the other is the low conversion efficiency of the strongly oxidized hydroxyl radical (•OH), which is produced by the decomposition of water due to high-energy X-ray radiation. These factors significantly limit the clinical effectiveness of radiotherapy.

Results: To address these limitations, we developed a highly stable and efficient nanoplatform (MnO2/Pt@BSA). Compared to MnO2@BSA, this platform demonstrates high stability, a high yield of oxygen (O2), enhanced production of •OH, and reduced clearance of •OH. The system exhibited increased O2 production in vitro and significantly improved oxygen production efficiency within 100 s at the Pt loading of 38.7%. Furthermore, compared with MnO2, the expression rate of hypoxia-inducible factor (HIF-1α) in glioma cells treated with MnO2/Pt decreased by half. Additionally, the system promotes •OH generation and consumes glutathione (GSH), thereby inhibiting the clearance of •OH and enhancing its therapeutic effect. Moreover, the degradation of the nanoplatform produces Mn2+, which serves as a magnetic resonance imaging (MRI) contrast agent with a T1-weighted enhancement effect at the tumor site. The nanoplatform exhibited excellent biocompatibility and performed multiple functions related to radiotherapy, with simpler components. In U87 tumor bearing mice model, we utilized MnO2/Pt nanocatalysis to enhance the therapeutic effect of radiotherapy on GBM.

Conclusion: This approach represents a novel and effective strategy for enhancing radiotherapy in gliomas, thereby advancing the field of catalytic radiotherapy and glioma treatment.

Keywords: MnO2/Pt@BSA nanoplatform; glioblastoma; nanocatalysis; radiotherapy.

MeSH terms

  • Animals
  • Brain Neoplasms* / diagnostic imaging
  • Brain Neoplasms* / metabolism
  • Brain Neoplasms* / radiotherapy
  • Cell Line, Tumor
  • Glioblastoma* / diagnostic imaging
  • Glioblastoma* / metabolism
  • Glioblastoma* / radiotherapy
  • Humans
  • Hydroxyl Radical* / metabolism
  • Magnetic Resonance Imaging* / methods
  • Manganese Compounds* / chemistry
  • Manganese Compounds* / pharmacology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Nanoparticles / chemistry
  • Oxides* / chemistry
  • Oxides* / pharmacology
  • Oxygen* / chemistry
  • Oxygen* / metabolism
  • Platinum / chemistry
  • Platinum / pharmacology
  • Tumor Microenvironment / drug effects

Substances

  • Manganese Compounds
  • Hydroxyl Radical
  • Oxides
  • Oxygen
  • Platinum
  • manganese dioxide
  • manganese oxide