A Distinct Genotype, D/Ep6, Detected in Korean Female Patients: Chlamydia trachomatis Characterization from 2017 - 2018

Clin Lab. 2024 Nov 1;70(11). doi: 10.7754/Clin.Lab.2024.240609.

Abstract

Background: The objective of this study lies in identifying the dominant genotype of C. trachomatis isolated in Republic of Korea (ROK) between 2017 and 2018.

Methods: A total of 504 clinical cervicovaginal swabs from patients were collected and inoculated on McCoy cell monolayers to isolate Chlamydial agents. C. trachomatis isolates were analyzed by sequencing of its ompA gene.

Results: A total of 54 C. trachomatis isolates were obtained. The constructed phylogenetic tree revealed the genotypes of isolates are D/Ep6 (48, 88.89%), D/Ep6-like (5, 9.26%), and Ja (1, 1.85%). The C. trachomatis D/Ep6-like have one single nucleotide polymorphism (SNP) compared to C. trachomatis D/Ep6, leading to E870K amino acid change.

Conclusions: Phylogenetic analysis demonstrated globally rare C. trachomatis D/Ep6 was dominant in the ROK from 2017 to 2018. Findings in this study act as a keystone, bridging past and present molecular epidemiology of C. trachomatis in context of ROK.

MeSH terms

  • Adult
  • Bacterial Outer Membrane Proteins* / genetics
  • Chlamydia Infections* / diagnosis
  • Chlamydia Infections* / epidemiology
  • Chlamydia Infections* / microbiology
  • Chlamydia trachomatis* / classification
  • Chlamydia trachomatis* / genetics
  • Chlamydia trachomatis* / isolation & purification
  • Female
  • Genotype*
  • Humans
  • Phylogeny*
  • Polymorphism, Single Nucleotide
  • Republic of Korea / epidemiology

Substances

  • Bacterial Outer Membrane Proteins
  • OMPA outer membrane proteins