Genetic diversity and antibiotic resistance patterns of Escherichia coli isolates causing septicemia: A phylogenetic typing and PFGE analysis

Mahshid Vakili; Hamidreza Goli; Javad Javidnia; Tahereh Alipour; Majid Eslami

doi:10.1016/j.diagmicrobio.2024.116586

Genetic diversity and antibiotic resistance patterns of Escherichia coli isolates causing septicemia: A phylogenetic typing and PFGE analysis

Diagn Microbiol Infect Dis. 2024 Oct 29;111(1):116586. doi: 10.1016/j.diagmicrobio.2024.116586. Online ahead of print.

Authors

Mahshid Vakili¹, Hamidreza Goli², Javad Javidnia³, Tahereh Alipour⁴, Majid Eslami⁵

Affiliations

¹ Reference Laboratory of Health, Semnan University of Medical Sciences, Semnan, Iran; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Students' Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.
² Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
³ Invasive Fungi Research Centre (IFRC), Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
⁴ Nervous System Stem Cell Research Center, Semnan University of Medical Sciences, Semnan, Iran.
⁵ Department of Bacteriology and Virology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran; Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran. Electronic address: [email protected].

PMID: 39522361
DOI: 10.1016/j.diagmicrobio.2024.116586

Abstract

Introduction: This study aims to analyze clinical isolates of E. coli causing septicemia across various phylogroups utilizing the PFGE method.

Materials and methods: A total of 100 clinical isolates were collected. The presence of CTX-M, TEM, SHV, KPC, MBL and OXA-48 genes was detected by PCR. Additionally, phylotyping, serotyping, and virulence-typing assay were done by PCR and PFGE methods to investigate the genetic diversity of the isolates.

Results: The O1 serotype and the HlyA gene were the most prevalent serotype and virulence gene, respectively. Notably, 34% of the isolates harbored SHV, TEM, and CTX-M-1 β-lactamase genes. All isolates showed resistance to amoxicillin and tetracycline, but no resistance to fosfomycin was seen. The most and least common phylotypes, according to PFGE analysis, belonged to phylogroups B2 and B1, respectively.

Conclusion: The data offers valuable insights into the genetic diversity and antibiotic resistance patterns of E. coli isolates responsible for septicemia.

Keywords: Escherichia coli isolates; Phylogenetic diversity; Septicemia patients.