Mechano-gradients drive morphogen-noise correction to ensure robust patterning

Sci Adv. 2024 Nov 15;10(46):eadp2357. doi: 10.1126/sciadv.adp2357. Epub 2024 Nov 15.

Abstract

Morphogen gradients instruct cells to pattern tissues. Although the mechanisms by which morphogens transduce chemical signals have been extensively studied, the roles and regulation of the physical communication between morphogen-receiver cells remain unclear. Here, we show that the Wnt/β-catenin-morphogen gradient, which patterns the embryonic anterior-posterior (AP) axis, generates intercellular tension gradients along the AP axis by controlling membrane cadherin levels in zebrafish embryos. This "mechano-gradient" is used for the cell competition-driven correction of noisy morphogen gradients. Naturally and artificially generated unfit cells, producing noisy Wnt/β-catenin gradients, induce local deformation of the mechano-gradients that activate mechanosensitive calcium channels in the neighboring fit cells, which then secrete annexin A1a to kill unfit cells. Thus, chemo-mechanical interconversion-mediated competitive communication between the morphogen-receiver cells ensures precise tissue patterning.

MeSH terms

  • Animals
  • Body Patterning*
  • Cadherins* / genetics
  • Cadherins* / metabolism
  • Embryo, Nonmammalian / metabolism
  • Mechanotransduction, Cellular
  • Morphogenesis
  • Wnt Signaling Pathway
  • Zebrafish Proteins / genetics
  • Zebrafish Proteins / metabolism
  • Zebrafish* / embryology
  • beta Catenin / genetics
  • beta Catenin / metabolism

Substances

  • Cadherins
  • Zebrafish Proteins
  • beta Catenin