Carboxymethyl cellulose-induced Cryptococcus laurentii improves disease resistance and regulates phenylpropane and reactive oxygen metabolism in grapefruit

Le Yang; Diantong Ma; Fang Wang; Li Liu; Lin Chen; Xiahong He; Junrong Tang; Jia Deng

doi:10.1016/j.foodchem.2024.141955

Carboxymethyl cellulose-induced Cryptococcus laurentii improves disease resistance and regulates phenylpropane and reactive oxygen metabolism in grapefruit

Food Chem. 2024 Nov 12;465(Pt 1):141955. doi: 10.1016/j.foodchem.2024.141955. Online ahead of print.

Authors

Le Yang¹, Diantong Ma², Fang Wang³, Li Liu⁴, Lin Chen³, Xiahong He⁵, Junrong Tang⁶, Jia Deng⁷

Affiliations

¹ Forestry College, Southwest Forestry University, Kunming 650224, PR China; Forestry College, Xingyang Agriculture and Forestry University, Xinyang 464000, PR China.
² Qiandongnan Institute of Forestry, Kaili 556003, PR China.
³ Forestry College, Southwest Forestry University, Kunming 650224, PR China; Key Laboratory of State Forestry Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, PR China.
⁴ Forestry College, Southwest Forestry University, Kunming 650224, PR China.
⁵ Forestry College, Southwest Forestry University, Kunming 650224, PR China; Yunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource, Southwest Forestry University, Kunming 650224, PR China. Electronic address: [email protected].
⁶ Forestry College, Southwest Forestry University, Kunming 650224, PR China; Key Laboratory of State Forestry Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, PR China. Electronic address: [email protected].
⁷ Forestry College, Southwest Forestry University, Kunming 650224, PR China; Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, PR China. Electronic address: [email protected].

PMID: 39550964
DOI: 10.1016/j.foodchem.2024.141955

Abstract

Green mould disease poses a significant threat to the citrus industry. Cryptococcus laurentii can stimulate the fruit defence system, whereas the use of antagonistic yeast alone demonstrates limited efficacy. This study investigated the molecular mechanisms of C. laurentii cultured with carboxymethyl cellulose (CMCC. laurentii), and evaluated the effects of CMCC. laurentii on phenylpropane and reactive oxygen metabolism in grapefruit fruit. Transcriptome analysis revealed that the upregulation of gene expression associated with yeast growth and antagonistic ability occurred in CMCC. laurentii after 72 h cultivation. Meanwhile, CMCC. laurentii reduced lesion diameter and disease incidence in fruit. This treatment promoted phenylpropane metabolism by activating PAL, C4H, 4CL, POD, and PPO and increasing the secondary metabolites. CMCC. laurentii also activated the AsA-GSH cycle, enhanced the activities of SOD and CAT, and reduced the accumulation of H₂O₂ and O₂^•-. The results suggested that CMCC. laurentii maintained high postharvest fruit quality in grapefruit fruit by elevating the phenylpropane and reactive oxygen metabolism.

Keywords: Biocontrol; Carboxymethyl cellulose; Cryptococcus laurentii; Grapefruit; Induced disease resistance; Penicillium digitatum; Transcriptomics.