Regional and longitudinal dynamics of human milk protein components assessed by proteome analysis on a fast and robust micro-flow LC-MS/MS system

Junxia Cao; Xinling Cui; Hai Lu; Hui Wang; Wen Ma; Zhan Yue; Kemiao Zhen; Qiaosi Wei; Hongmei Li; Shilong Jiang; Wantao Ying

doi:10.1016/j.foodchem.2024.141981

Regional and longitudinal dynamics of human milk protein components assessed by proteome analysis on a fast and robust micro-flow LC-MS/MS system

Food Chem. 2024 Nov 9;465(Pt 1):141981. doi: 10.1016/j.foodchem.2024.141981. Online ahead of print.

Authors

Junxia Cao¹, Xinling Cui², Hai Lu³, Hui Wang⁴, Wen Ma⁵, Zhan Yue⁶, Kemiao Zhen⁶, Qiaosi Wei⁷, Hongmei Li⁸, Shilong Jiang⁹, Wantao Ying¹⁰

Affiliations

¹ School of Basic Medical Science, Anhui Medical University, Hefei 230032, PR China; State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, PR China.
² Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, PR China; Department of Bioengineering, Beijing Technology and Business University, Beijing 100048, PR China.
³ Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, PR China.
⁴ State Key Laboratory of Systems Medicine for Cancer, Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China.
⁵ State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China.
⁶ State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, PR China.
⁷ Feihe Research Institute, Heilongjiang Feihe Dairy Co., Ltd, Beijing 100016, PR China.
⁸ Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, PR China. Electronic address: [email protected].
⁹ Feihe Research Institute, Heilongjiang Feihe Dairy Co., Ltd, Beijing 100016, PR China; C-16(th) FL,Star City, No10, Jiuxianqiao Rd, Chaoyang District, Beijing, 100016, PR China. Electronic address: [email protected].
¹⁰ School of Basic Medical Science, Anhui Medical University, Hefei 230032, PR China; State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, PR China. Electronic address: [email protected].

PMID: 39550967
DOI: 10.1016/j.foodchem.2024.141981

Abstract

An in-depth exploration of molecular composition of human milk could provide a scientific basis for the development of substitutes. The present study was conducted to analyze human milk proteins from 110 individuals from five regions of China and across three stages of lactation to investigate the change patterns. We developed a micro-flow liquid chromatography tandem mass spectrometry (μLC-MS/MS) system with data-independent acquisition (DIA) proteomics technology that can rapidly and stably characterize the human milk proteome. In total, 2796 proteins were identified. Among these proteins, CPM, ACSL1, and RPL13 changed significantly during lactation, and SCP2, GALK1 and GALE changed significantly between regions. Bioinformatics analysis revealed that human milk is altered by complex interactions between genetic and environmental factors. Our results not only reveal the regional and longitudinal patterns of change in human milk proteome but also provide theoretical basis and technical support for the production and quality control of infant formula.

Keywords: DIA; Human milk proteomics; Large-scale analysis; μLC–MS/MS.