Genome-wide linkage mapping of Fusarium crown rot in common wheat (Triticum aestivum L.)

Faji Li; Can Guo; Qi Zhao; Weie Wen; Shengnan Zhai; Xinyou Cao; Cheng Liu; Dungong Cheng; Jun Guo; Yan Zi; Aifeng Liu; Jianmin Song; Jianjun Liu; Jindong Liu; Haosheng Li

doi:10.3389/fpls.2024.1457437

Genome-wide linkage mapping of Fusarium crown rot in common wheat (Triticum aestivum L.)

Front Plant Sci. 2024 Nov 1:15:1457437. doi: 10.3389/fpls.2024.1457437. eCollection 2024.

Authors

Faji Li¹, Can Guo², Qi Zhao³, Weie Wen⁴, Shengnan Zhai¹, Xinyou Cao¹, Cheng Liu¹, Dungong Cheng¹, Jun Guo¹, Yan Zi¹, Aifeng Liu¹, Jianmin Song¹, Jianjun Liu¹, Jindong Liu⁵, Haosheng Li¹

Affiliations

¹ Crop Research Institute, National Engineering Laboratory for Wheat and Maize, National Key Laboratory of Wheat Improvement, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan, China.
² Shangqiu Academy of Agriculture and Forestry Sciences, Shangqiu, China.
³ Collage of Life Science, Yantai University, Yantai, China.
⁴ Department of Cell Biology, Zunyi Medical University, Zunyi, Guizhou, China.
⁵ Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.

Abstract

Introduction: Powdery mildew (PM) poses an extreme threat to wheat yields and quality.[Methods] In this study, 262 recombinant inbred lines (RILs) of Doumai and Shi 4185 cross were used to map PM resistance genes across four environments. A high-density genetic linkage map of the Doumai/Shi 4185 RIL population was constructed using the wheat Illumina iSelect 90K single-nucleotide polymorphism (SNP) array.

Results: In total, four stable quantitative trait loci (QTLs) for PM resistance, QPm.caas-2AS, QPm.caas-4AS, QPm.caas-4BL, and QPm.caas-6BS, were detected and explained 5.6%-15.6% of the phenotypic variances. Doumai contributed all the resistance alleles of QPm.caas-2AS, QPm.caas-4AS, QPm.ca as-4BL, and QPm.caas-6BS. Among these, QPm.caas-4AS and QPm.caas-6BS overlapped with the previously reported loci, whereas QPm.caas-2AS and QPm.caas-4BL are potentially novel. Additionally, six high-confidence genes encoding the NBS-LRR-like resistance protein, disease resistance protein family, and calcium/calmodulin-dependent serine/threonine-kinase were selected as the candidate genes for PM resistance. Three kompetitive allele-specific PCR (KASP) markers, Kasp_PMR_2AS for QPm.caas-2AS, Kasp_PMR_4BL for QPm.caas-4BL, and Kasp_PMR_6BS for QPm.caas-6BS, were developed, and their genetic effects were validated in a natural population including 100 cultivars.

Discussion: These findings will offer valuable QTLs and available KASP markers to enhance wheat marker-assisted breeding for PM resistance.

Keywords: Fusarium crown rot (FCR); common wheat; kompetitive allele-specific PCR (KASP); molecular marker-assisted selection; quantitative trait Loci (QTL).

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was funded by the Focus on Research and Development Plan in Shandong Province (2022LZGC005), National Major Agricultural Science and Technology Project (NK20220604), Taishan Scholar Program of Shandong (tspd20221108), Natural Science Foundation of Shandong Province (ZR2023205), and China Agriculture Research System (CARS-03-6).