Improved circuitry and post-processing for interleaved fast-scan cyclic voltammetry and electrophysiology measurements

The combination of electrophysiology and electrochemistry acquisition methods using a single carbon fiber microelectrode (CFM) in the brain has enabled more extensive analysis of neurochemical release, neural activity, and animal behavior. Predominantly, analog CMOS (Complementary Metal Oxide Semiconductor) switches are used for these interleaved applications to alternate the CFM output between electrophysiology and electrochemistry acquisition circuitry. However, one underlying issue with analog CMOS switches is the introduction of transient voltage artifacts in recorded electrophysiology signals resulting from CMOS charge injection. These injected artifacts attenuate electrophysiology data and delay reliable signal observation after every switch actuation from electrochemistry acquisition. Previously published attempts at interleaved electrophysiology and electrochemistry were able to recover reliable electrophysiology data within approximately 10-50 ms after switch actuation by employing various high-pass filtering methods to mitigate the observed voltage artifacts. However, high-pass filtering of this nature also attenuates valuable portions of the local-field potential (LFP) frequency range, thus limiting the extent of network-level insights that can be derived from in vivo measurements. This paper proposes a solution to overcome the limitation of charge injection artifacts that affect electrophysiological data while preserving important lower-frequency LFP bands. A voltage follower operational amplifier was integrated before the CMOS switch to increase current flow to the switch and dissipate any injected charge. This hardware addition resulted in a 16.98% decrease in electrophysiology acquisition delay compared to circuitry without a voltage follower. Additionally, single-term exponential modeling was implemented in post-processing to characterize and subtract remaining transient voltage artifacts in recorded electrophysiology data. As a result, electrophysiology data was reliably recovered 3.26 ± 0.22 ms after the beginning of the acquisition period (a 60% decrease from previous studies), while also minimizing LFP attenuation. Through these advancements, coupled electrophysiology and electrochemistry measurements can be conducted at higher scan rates while retaining data integrity for a more comprehensive analysis of neural activity and neurochemical release.