Abstract
The initial adhesive interactions between circulating leukocytes and endothelia are mediated, in part, by P-selectin. We now report the expression cloning of a functional ligand for P-selectin from an HL-60 cDNA library. The predicted amino acid sequence reveals a novel mucin-like transmembrane protein. Significant binding of transfected COS cells to P-selectin requires coexpression of both the protein ligand and a fucosyltransferase. This binding is calcium dependent and can be inhibited by a neutralizing monoclonal antibody to P-selectin. Cotransfected COS cells express the ligand as a homodimer of 220 kd. A soluble ligand construct, when coexpressed with fucosyltransferase in COS cells, also mediates P-selectin binding and is immunocrossreactive with the major HL-60 glycoprotein that specifically binds P-selectin.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Blotting, Southern
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CHO Cells
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Cell Adhesion
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Cell Adhesion Molecules / metabolism
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Cell Line
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Cloning, Molecular
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Cricetinae
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DNA, Complementary / metabolism
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Endothelium, Vascular / physiology
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Gene Library
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Humans
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Leukemia, Promyelocytic, Acute
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Leukocytes / physiology
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Macromolecular Substances
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Membrane Glycoproteins / biosynthesis*
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Membrane Glycoproteins / metabolism
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Molecular Sequence Data
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P-Selectin
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Platelet Membrane Glycoproteins / metabolism*
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Sequence Homology, Amino Acid
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Transfection
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Tumor Cells, Cultured
Substances
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Cell Adhesion Molecules
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DNA, Complementary
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Macromolecular Substances
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Membrane Glycoproteins
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P-Selectin
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P-selectin ligand protein
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Platelet Membrane Glycoproteins
Associated data
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GENBANK/L20821
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GENBANK/L20822
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GENBANK/L20823
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GENBANK/L20888
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GENBANK/L20889
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GENBANK/L33709
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GENBANK/S60904
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GENBANK/S60905
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GENBANK/S60924
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GENBANK/U02297