Abstract
Absorption of recombinant human granulocyte colony-stimulating factors (rhG-CSF) following intranasal administration of aqueous preparations containing rhG-CSF with or without dimethyl-beta-cyclodextrin was investigated. It was found that rhG-CSF was absorbed and the total leukocyte numbers in peripheral blood markedly increased after intranasal instillation of rhG-CSF solution (pH 4) without excipients. Simultaneously administered dimethyl-beta-cyclodextrin enhanced nasal absorption of rhG-CSF. Consequently, serum rhG-CSF concentrations and total leukocyte numbers were greatly increased.
MeSH terms
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Administration, Intranasal
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Animals
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Cyclodextrins / administration & dosage
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Cyclodextrins / pharmacology
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Granulocyte Colony-Stimulating Factor / administration & dosage
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Granulocyte Colony-Stimulating Factor / blood
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Granulocyte Colony-Stimulating Factor / pharmacokinetics
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Growth Substances / administration & dosage
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Growth Substances / pharmacology
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Humans
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Leukocyte Count / drug effects*
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Male
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Rabbits
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Recombinant Proteins / administration & dosage
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Recombinant Proteins / pharmacokinetics
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beta-Cyclodextrins*
Substances
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Cyclodextrins
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Growth Substances
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Recombinant Proteins
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beta-Cyclodextrins
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Granulocyte Colony-Stimulating Factor
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heptakis(2,6-O-dimethyl)beta-cyclodextrin