Abstract
Ten hepatitis B surface antigen seropositive carriers (5 asymptomatic and 5 with chronic liver disease) were tested for HBeAg/anti-HBe and for HBV-DNA using the polymerase chain reaction. Five were DNA-positive, 2 with HBeAg and 3 with anti-HBe. Nucleotide sequences were determined for these 5 cases. Hepatitis B virus DNA from one cirrhotic carrier with anti-HBe had a mutation in the precore region (nucleotide position 1862) which may affect signal peptide cleavage and HBeAg synthesis. In the other 2 anti-HBe- and DNA-positive cases, a cirrhotic carrier and an asymptomatic case, there was a mutation at nucleotide position 1896 leading to a termination codon in the precore region. In all 5 patients, except for one or two missense mutations, there was no significant variation in the core region.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Base Sequence
-
Carrier State / virology*
-
Consensus Sequence
-
DNA Primers / genetics
-
DNA, Viral / blood
-
DNA, Viral / genetics
-
Hepatitis B / virology*
-
Hepatitis B Antibodies / blood
-
Hepatitis B Core Antigens / genetics
-
Hepatitis B Surface Antigens / blood
-
Hepatitis B e Antigens / blood
-
Hepatitis B virus / genetics*
-
Hepatitis B virus / isolation & purification
-
Hepatitis, Chronic / virology*
-
Humans
-
Indien
-
Molecular Sequence Data
-
Mutation*
-
Open Reading Frames
-
Sequence Homology, Amino Acid
-
Sequence Homology, Nucleic Acid
Substances
-
DNA Primers
-
DNA, Viral
-
Hepatitis B Antibodies
-
Hepatitis B Core Antigens
-
Hepatitis B Surface Antigens
-
Hepatitis B e Antigens
Associated data
-
GENBANK/Z68139
-
GENBANK/Z68140
-
GENBANK/Z68141
-
GENBANK/Z68142
-
GENBANK/Z68143