Abstract
The human beta-catenin locus (CTNNB1) was mapped by in situ fluorescence analysis to band p21 on the short arm of chromosome 3, a region frequently affected by somatic alterations in a variety of tumors. PCR primers for the genomic amplification of beta-catenin sequences were selected on the basis of homology to exon 4 of the Drosophila armadillo gene. Analysis of a panel of somatic cell hybrids confirmed the localization of beta-catenin on human chromosome 3. Furthermore, exclusion mapping of three hybrids carrying defined fragments of the short arm of human chromosome 3 allowed us to determine the position of the CTNNB1 locus close to the marker D3S2 in 3p21.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenomatous Polyposis Coli Protein
-
Animals
-
Armadillo Domain Proteins
-
Base Sequence
-
Chromosome Mapping
-
Chromosomes, Human, Pair 3*
-
Cytoskeletal Proteins / genetics*
-
Cytoskeletal Proteins / physiology
-
Drosophila Proteins*
-
Drosophila melanogaster / genetics
-
Genes
-
Humans
-
Hybrid Cells
-
In Situ Hybridization, Fluorescence
-
Mice
-
Molecular Sequence Data
-
Neoplasms / genetics*
-
Polymerase Chain Reaction
-
Proteins / genetics
-
Sequence Homology, Nucleic Acid
-
Species Specificity
-
Trans-Activators*
-
Transcription Factors
-
beta Catenin
Substances
-
ARM protein, Drosophila
-
Adenomatous Polyposis Coli Protein
-
Armadillo Domain Proteins
-
CTNNB1 protein, human
-
CTNNB1 protein, mouse
-
Cytoskeletal Proteins
-
Drosophila Proteins
-
Proteins
-
Trans-Activators
-
Transcription Factors
-
beta Catenin