Vitamin D status modulates rat colonic M3 muscarinic receptor characteristics and coupling to guanylate cyclase

FEBS Lett. 1994 Jun 20;347(1):27-30. doi: 10.1016/0014-5793(94)00498-6.

Abstract

The present studies were conducted to determine whether [3H]quinuclidinyl benzilate binding in rat colonic membranes and/or carbachol-mediated stimulation of particulate guanylate cyclase were altered by changes in vitamin D status. EC50 values for the stimulation of colonic guanylate cyclase by carbachol were found to be significantly greater in vitamin D-deficient rats compared to their D-sufficient counterparts. Concomitantly, the density of receptors (Bmax) were significantly lower, and dissociation constants (Kd) were significantly higher in D-deficient colonic membranes. In vitamin D-repleted animals, moreover, all of these aforementioned alterations were at least partially corrected.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Atropine / pharmacology
  • Colon / metabolism*
  • Diamines / pharmacology
  • Enzyme Activation
  • Guanylate Cyclase / metabolism*
  • Male
  • Muscarinic Antagonists
  • Nutritional Status
  • Parasympatholytics / pharmacology
  • Piperidines / pharmacology
  • Pirenzepine / pharmacology
  • Quinuclidinyl Benzilate / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Muscarinic / metabolism*
  • Vitamin D / physiology*

Substances

  • Diamines
  • Muscarinic Antagonists
  • Parasympatholytics
  • Piperidines
  • Receptors, Muscarinic
  • Vitamin D
  • Pirenzepine
  • Quinuclidinyl Benzilate
  • Atropine
  • 4-diphenylacetoxy-1,1-dimethylpiperidinium
  • Guanylate Cyclase
  • methoctramine