Abstract
The present studies were conducted to determine whether [3H]quinuclidinyl benzilate binding in rat colonic membranes and/or carbachol-mediated stimulation of particulate guanylate cyclase were altered by changes in vitamin D status. EC50 values for the stimulation of colonic guanylate cyclase by carbachol were found to be significantly greater in vitamin D-deficient rats compared to their D-sufficient counterparts. Concomitantly, the density of receptors (Bmax) were significantly lower, and dissociation constants (Kd) were significantly higher in D-deficient colonic membranes. In vitamin D-repleted animals, moreover, all of these aforementioned alterations were at least partially corrected.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Atropine / pharmacology
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Colon / metabolism*
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Diamines / pharmacology
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Enzyme Activation
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Guanylate Cyclase / metabolism*
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Male
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Muscarinic Antagonists
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Nutritional Status
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Parasympatholytics / pharmacology
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Piperidines / pharmacology
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Pirenzepine / pharmacology
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Quinuclidinyl Benzilate / metabolism*
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Rats
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Rats, Sprague-Dawley
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Receptors, Muscarinic / metabolism*
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Vitamin D / physiology*
Substances
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Diamines
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Muscarinic Antagonists
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Parasympatholytics
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Piperidines
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Receptors, Muscarinic
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Vitamin D
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Pirenzepine
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Quinuclidinyl Benzilate
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Atropine
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4-diphenylacetoxy-1,1-dimethylpiperidinium
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Guanylate Cyclase
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methoctramine