Abstract
Members of a family of highly conserved proteins, termed 14-3-3 proteins, were found by several experimental approaches to associate with Raf-1, a central component of a key signal transduction pathway. Optimal complex formation required the amino-terminal regulatory domain of Raf-1. The association of 14-3-3 proteins and Raf-1 was not substantially affected by the activation state of Raf.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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14-3-3 Proteins
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3T3 Cells
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Animals
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Binding Sites
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Cell Line
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Enzyme Activation
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Humans
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Mice
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Nerve Tissue Proteins / metabolism
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Phosphorylation
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Poly(ADP-ribose) Polymerases / metabolism
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Protein Serine-Threonine Kinases / metabolism*
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Proteins / chemistry
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Proteins / metabolism*
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-raf
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Signal Transduction*
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Spodoptera
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Tyrosine 3-Monooxygenase*
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Zinc Fingers
Substances
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14-3-3 Proteins
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Nerve Tissue Proteins
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Proteins
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Proto-Oncogene Proteins
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Tyrosine 3-Monooxygenase
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Poly(ADP-ribose) Polymerases
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Protein Serine-Threonine Kinases
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Proto-Oncogene Proteins c-raf