Molecular and cellular characterization of the GABAA receptor in the rat pancreas

Mol Cell Endocrinol. 1994 Jul;103(1-2):157-63. doi: 10.1016/0303-7207(94)90083-3.

Abstract

In the present study, we characterize the molecular structure of the GABAA receptor in pancreas, islets, alpha and beta cells, and in RIN 1046-38 cells. Using the polymerase chain reaction and specific primers for 11 out of the 15 subunits known so far, that may contribute to the composition of the GABAA receptors, we demonstrate that pancreas and its cellular components, as well RIN 1046-38 cells, might contain a GABAA receptor resulting from all the possible combinations in a pentameric configuration of the subtypes alpha 1, alpha 2, alpha 3 of the alpha subunit family, beta 1, beta 2, beta 3 subtypes of the beta subunit family, delta subunit and gamma 2 subtype of the gamma subunit family. The presence of the gamma 2 subunit renders the GABAA receptors potentially sensitive to allosteric modulators.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Brain / metabolism
  • Gene Expression
  • Insulinoma / metabolism
  • Islets of Langerhans / metabolism
  • Male
  • Molecular Sequence Data
  • Pancreas / metabolism*
  • Pancreatic Neoplasms / metabolism
  • Polymerase Chain Reaction
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, GABA / genetics*
  • Tumor Cells, Cultured

Substances

  • RNA, Messenger
  • Receptors, GABA