Abstract
The CD26 activation antigen (Ag) which is expressed on a subpopulation of human T cells has been characterized as dipeptidyl peptidase IV (DPP IV, EC 3.4.14.5). In this paper, we describe the antibody binding profile of CD26/DPP IV, purified from human peripheral blood lymphocytes. The purified molecule binds to the anti-Ta1, anti-1F7 and anti-134-2C2 monoclonal antibodies (mAb), reported to react with cell-bound CD26 Ag. Among unclustered mAb recognizing T cell antigens, two, anti-BT5/9 and anti-TA5.9 were found to react with purified and cell-bound CD26 Ag. The classification of the BT5/9 Ag, the functional properties of the BT5/9+ T cell subset, as well as the in vivo effect of anti-BT5/9 mAb administration, are re-interpreted in the light of its specificity. Applying the anti-TA5.9 mAb in three color FACS analyses, we demonstrated that CD26+bright cells co-express CD45RO but not HLA-DR and CD38.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / immunology
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Antibodies, Monoclonal / metabolism*
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Antigen-Antibody Reactions
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Antigens, CD / immunology
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Antigens, CD / isolation & purification
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Antigens, CD / metabolism*
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Antigens, Differentiation, T-Lymphocyte / immunology
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Antigens, Differentiation, T-Lymphocyte / isolation & purification
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Antigens, Differentiation, T-Lymphocyte / metabolism*
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Binding Sites, Antibody
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Chromatography, Affinity
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Dipeptidyl Peptidase 4
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Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / immunology
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Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / isolation & purification
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Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / metabolism*
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Humans
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T-Lymphocytes / enzymology*
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T-Lymphocytes / immunology*
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Differentiation, T-Lymphocyte
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Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
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Dipeptidyl Peptidase 4