Ras transformation alters the composition of extracellular matrix proteoglycans in rat fibroblasts

Biochem Biophys Res Commun. 1994 Apr 29;200(2):925-32. doi: 10.1006/bbrc.1994.1538.

Abstract

ts371 KiMuSV-transformed rat kidney fibroblasts growing at the permissive temperature to allow the expression of a transforming p21 ras protein secrete a different pattern of chondroitin/dermatan sulfate proteoglycans than when growing at the non-permissive temperature. Transformed cells produce less versican and larger amounts of biglycan and decorin than their normal counterpart, and they do not secrete 35S-labeled PG-100. Western blot experiments showed the presence of PG-100 epitope, suggesting that the apparent effect of the oncoprotein is due to a reduced sulfation of the molecule. Transformed and non-transformed cells are similarly sensitive to the proteoglycan-induction effect of TGF-beta.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biglycan
  • Cell Transformation, Neoplastic / genetics
  • Cells, Cultured
  • Chondroitin Sulfate Proteoglycans / metabolism
  • Decorin
  • Extracellular Matrix Proteins / metabolism*
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Genes, ras*
  • Lectins, C-Type
  • Proteoglycans / metabolism*
  • Rats
  • Temperature
  • Transforming Growth Factor beta / pharmacology
  • Versicans

Substances

  • Bgn protein, rat
  • Biglycan
  • Chondroitin Sulfate Proteoglycans
  • Dcn protein, rat
  • Decorin
  • Extracellular Matrix Proteins
  • Lectins, C-Type
  • Proteoglycans
  • Transforming Growth Factor beta
  • Vcan protein, rat
  • Versicans