Abstract
To identify the gene or genes associated with insulin resistance in Type II (non-insulin-dependent) diabetes mellitus, subtraction libraries were prepared from skeletal muscle of normal and diabetic humans and screened with subtracted probes. Only one clone out of 4000 was selectively overexpressed in Type II diabetic muscle as compared to muscle of non-diabetic or Type I diabetic individuals. This clone encoded a new 29-kilodalton member of the Ras-guanosine triphosphatase superfamily and was termed Rad (Ras associated with diabetes). Messenger ribonucleic acid of Rad was expressed primarily in skeletal and cardiac muscle and was increased an average of 8.6-fold in the muscle of Type II diabetics as compared to normal individuals.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Blotting, Northern
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Blotting, Southern
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Chromosome Aberrations
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Cloning, Molecular
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Diabetes Mellitus, Type 2 / genetics*
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Diabetes Mellitus, Type 2 / metabolism
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GTP Phosphohydrolases / biosynthesis
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GTP Phosphohydrolases / chemistry
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GTP Phosphohydrolases / genetics
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GTP-Binding Proteins / biosynthesis
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GTP-Binding Proteins / chemistry
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GTP-Binding Proteins / genetics*
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Gene Amplification
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Genes*
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Humans
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Insulin Resistance / genetics*
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Molecular Sequence Data
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Muscles / metabolism*
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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ras Proteins*
Substances
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RNA, Messenger
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RRAD protein, human
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GTP Phosphohydrolases
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GTP-Binding Proteins
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ras Proteins