Abstract
An endometrioid ovarian adenocarcinoma cell line CAVEOC-2 was characterized. Maintained in monolayered culture, CAVEOC-2 cells exhibited a 33-hr doubling time. When xenografted into nude mice, these cells produced fast growing tumors. Colony-forming efficiency in agar was 50%. DNA index was 1.5 and cytogenetic analysis showed a triploid karyotype. CAVEOC-2 cells did not express mdr-1 gene and were chemosensitive to doxorubicin (IC50 = 1.82 +/- 0.76 mumol/l), paclitaxel (IC50 = 3.33 +/- 0.26 nmol/l) and docetaxel (IC50 = 0.68 +/- 0.28 nmol/l), while they showed an intermediate sensitivity to cisplatin (IC50 = 9.40 +/- 1.02 mumol/l). CAVEOC-2 cells seemed highly radioresistant (SF2 = 0.81, alpha = 0.02 Gy-1, beta = 0.025 Gy2, and MID = 4.31 Gy). Activities of glutathione S transferase and gamma-glutamyl transpeptidase were respectively 23.5- and 3.4- fold higher than those of sensitive A2780 cell line. These characteristics make the CAVEOC-2 cells a suitable model for the study of human endometrioid ovarian adenocarcinoma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
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Animals
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Antineoplastic Agents, Phytogenic / pharmacology*
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Base Sequence
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Carcinoma, Endometrioid / drug therapy*
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Carcinoma, Endometrioid / pathology*
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Carcinoma, Endometrioid / radiotherapy
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Cell Division / drug effects
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DNA, Neoplasm / analysis
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DNA, Neoplasm / genetics
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Docetaxel
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Doxorubicin / pharmacology
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Drug Screening Assays, Antitumor
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Female
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Flow Cytometry
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Gene Expression
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Glutathione Peroxidase / metabolism
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Glutathione Transferase / metabolism
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Humans
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Immunohistochemistry
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Karyotyping
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Mice
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Mice, Nude
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Middle Aged
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Molecular Sequence Data
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Ovarian Neoplasms / drug therapy*
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Ovarian Neoplasms / pathology*
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Ovarian Neoplasms / radiotherapy
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Paclitaxel / analogs & derivatives
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Paclitaxel / pharmacology
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Ploidies
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RNA, Messenger / metabolism
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Radiation Tolerance*
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Taxoids*
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Tumor Cells, Cultured
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gamma-Glutamyltransferase / metabolism
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Antineoplastic Agents, Phytogenic
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DNA, Neoplasm
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RNA, Messenger
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Taxoids
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Docetaxel
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Doxorubicin
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Glutathione Peroxidase
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gamma-Glutamyltransferase
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Glutathione Transferase
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Paclitaxel