Abstract
Infection of CD4+ T-lymphoblastoid cells by cytopathic strains of HIV-1 results in an increase in cell volume that leads to lysis and cell death. The increase in volume is attributable in part to an HIV-induced increase in intracellular monovalent ion concentrations mediated by the plasma membrane-associated Na+/K+/2 Cl- cotransporter. Loop diuretics, which inhibit cotransporter activity, blocked HIV-induced HIV production and cytopathic effects at physiologically employed concentrations.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antiviral Agents / pharmacology*
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CD4-Positive T-Lymphocytes / cytology
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CD4-Positive T-Lymphocytes / drug effects
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CD4-Positive T-Lymphocytes / virology
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Carrier Proteins / antagonists & inhibitors*
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Cell Death
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Cell Line
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Cytopathogenic Effect, Viral / drug effects
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Furosemide / pharmacology*
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HIV Core Protein p24 / metabolism
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HIV-1 / drug effects*
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HIV-1 / growth & development
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HIV-1 / metabolism
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Humans
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Membrane Proteins / antagonists & inhibitors*
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Potassium / metabolism
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Sodium / metabolism
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Sodium-Potassium-Chloride Symporters
Substances
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Antiviral Agents
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Carrier Proteins
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HIV Core Protein p24
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Membrane Proteins
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Sodium-Potassium-Chloride Symporters
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Furosemide
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Sodium
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Potassium