Abstract
The recombinant bacillus Calmette-Guérin (rBCG) secretion system utilizing an extracellular alpha antigen of Mycobacterium kansasii (alpha-K) was characterized biochemically and immunologically. The human immunodeficiency virus type 1 (HIV-1) p17gag B cell epitope fused to alpha-K was secreted in extremely large amounts. At least three mice out of seven inoculated with rBCG generated high titres of antibody to the epitope. The long-lasting antibody production persisted more than 14 months.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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BCG Vaccine / genetics
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BCG Vaccine / metabolism*
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Base Sequence
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Epitopes / genetics
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Epitopes / immunology
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Gene Products, gag / genetics
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Gene Products, gag / immunology
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Genetic Vectors / immunology*
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Genetic Vectors / metabolism*
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HIV Antibodies / biosynthesis
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HIV Antibodies / genetics
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HIV Antigens / genetics
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HIV Antigens / immunology
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HIV-1 / genetics
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HIV-1 / immunology
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Humans
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Recombinant Fusion Proteins / metabolism*
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Time Factors
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Vaccines, Synthetic / immunology*
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Vaccines, Synthetic / metabolism*
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Viral Proteins*
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gag Gene Products, Human Immunodeficiency Virus
Substances
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BCG Vaccine
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Epitopes
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Gene Products, gag
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HIV Antibodies
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HIV Antigens
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Recombinant Fusion Proteins
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Vaccines, Synthetic
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Viral Proteins
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gag Gene Products, Human Immunodeficiency Virus
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p17 protein, Human Immunodeficiency Virus Type 1